Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Stromelysin-2 (matrix metalloproteinase 10) is inducible in lymphoma cells and accelerates the growth of lymphoid tumors in vivo.

doi: 10.4049/jimmunol.173.6.3605

Figure Lengend Snippet: FIGURE 3. Up-regulation of MMP10 in lymphoma cells upon exposure to cytokines. Raji cells (A) and several B lymphoma cell lines (B) were stimulated with various cytokines, including IL-13, for 14 h. MMP10, MMP3, and TIMP-1 mRNA expression was then measured by RT-PCR, using HT1080 fibrosarcoma cell line as a positive control. Results are representative of at least three independent experiments. The expression of GAPDH was included as an RNA loading control. Results are representa- tive of at least two independent experiments.

Article Snippet: Recombinant human cytokines (IL-4, IL-6, IL-13, and IL-1) were purchased from R&D Systems (Minneapolis, MN).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Positive Control, Control

Journal: Communications Biology

Article Title: The HDAC inhibitor zabadinostat is a systemic regulator of adaptive immunity

doi: 10.1038/s42003-023-04485-y

Figure Lengend Snippet: a Schematic representation of the experiment with zabadinostat. Balb/c mice were treated with orally administrated zabadinostat at 25 mg/kg for 14 days (5 days on/2 days off) or vehicle only; n = 4 per group; b relative body weight representation of treated and non-treated mice; n = 4; results presented as mean values +/−SD. c General T-cell activation and CD4-positive T cells measured by flow cytometry; measurements were performed in pooled splenocytes from four mice; also, we noticed similar percentage of CD8-positive T cells in control and zabadinostat-treated groups (26.2% and 24.3%). The viability of the splenocytes was similar in zabadinostat treatment compare to untreated control (70.9% and 76.6% viable cells, respectively). d Analysis of panel of Th1, Th2, Th1/Th2, General Th, Th17 and Th9 cytokines in serum collected from mice treated with zabadinostat and the control group; n = 4; results presented as mean values +/−SD; Student’s t test.

Article Snippet: From this experiment, serum was collected from each mouse, and the panel of twelve cytokines (IFNγ, IL-2, IL-4, IL-5, IL-13, IL-10, IL-9, TNFα, IL-6, IL-17A, IL-17F, IL-22) was analysed using LEGENDplex MU Th Cytokine Panel (12-plex) VbP V03 according to manufacturer’s instruction (741044, Biolegend).

Techniques: Activation Assay, Flow Cytometry, Control